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Table of Contents
Vol. 23, No. 1-3, 2009
Issue release date: February 2009
Section title: Original Paper
Cell Physiol Biochem 2009;23:009–024

Expression of ENaC and other Transport Proteins in Xenopus Oocytes is Modulated by Intracellular Na+

Kusche-Vihrog K.1,* · Segal A.2,* · Grygorczyk R.3 · Bangel-Ruland N.1 · Van Driessche W.2 · Weber W.-M.1
1 Institute of Animal Physiology, Westphalian Wilhelms-University Muenster,2Laboratory of Physiology, KU Leuven, Campus Gasthuisberg, Leuven,3Research Centre, Centre hospitalier de l’Université de Montréal - Hôtel-Dieu, 3850 Saint-Urbain, Montréal,*These authors contributed equally
email Corresponding Author

Wolf-Michael Weber

Institute of Animal Physiology, WWU Muenster

Hindenburgplatz 55, 48143 Muenster (Germany)

Tel. +49 251 83 21782, Fax: +49 251 83 21785

E-Mail wmw@uni-muenster.de

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The expression of the epithelial Na+ channel (ENaC) is tissue-specific and dependent on a variety of mediators and interacting proteins. Here we examined the role of intracellular Na+ ([Na+]i) as a modulator of the expression of rat ENaC in Xenopus laevis oocytes. We manipulated [Na+]i of ENaC-expressing oocytes in the range of 0-20 mM by incubating in extracellular solutions of different [Na+]o. Electrophysiological, protein biochemical and fluorescence optical methods were used to determine the effects of different [Na+]i on ENaC expression and membrane abundance. In voltage-clamp experiments we found that amiloride-sensitive ENaC current (Iami) and conductance (Gami) peak at a [Na+]i of ∼10 mM Na+, but were significantly reduced in 5 mM and 20 mM [Na+]i. Fluorescence intensity of EGFP-ENaC-expressing oocytes also followed a bell-shaped curve with a maximum at ∼ 10 mM [Na+]i. In Western blot experiments with specific anti-ENaC antibodies the highest protein expression was found in ENaC-expressing oocytes with [Na+]i of 10-15 mM. Since ENaC is also highly permeable for Li+, we incubated ENaC-expressing oocytes in different Li+ concentrations and found a peak of Iami and Gami with 5 mM Li+. The influence of [Na+]i on the expression is not ENaC-specific, since expression of a Cl- channel (CFTR) and a Na+/glucose cotransporter (SGLT1) showed the same dependence on [Na+]i. We conclude that specific concentrations of Na+ and Li+ influence the expression and abundance of ENaC and other transport proteins in the plasma membrane in Xenopus laevis oocytes. Furthermore, we suggest the existence of a general mechanism dependent on monovalent cations that optimizes the expression of membrane proteins.

© 2009 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: November 07, 2008
Published online: February 18, 2009
Issue release date: February 2009

Number of Print Pages: 16
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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