Effects of Advanced Glycation End Products on Hyaluronan Photolysis: A New Mechanism of Diabetic Vitreopathy

Vol. 36, No. 6, 2004

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Original Paper

Effects of Advanced Glycation End Products on Hyaluronan Photolysis: A New Mechanism of Diabetic Vitreopathy
Chihiro Katsumura^a, Tetsuya Sugiyama^a, Kimitoshi Nakamura^a,b, Hiroshi Obayashi^c, Goji Hasegawa^c, Hidehiro Oku^a, Tsunehiko Ikeda^a

^aDepartment of Ophthalmology, Osaka Medical College, Takatsuki, Osaka,
^bNakamura Eye Clinic, Matsumoto, Nagano, and
^cFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan

Address of Corresponding Author

Ophthalmic Res 2004;36:327-331 (DOI: 10.1159/000081635)

Key Words

Advanced glycation end products
Diabetic retinopathy
Hyaluronan
Photosensitizer
High-performance liquid chromatography
Diabetic vitreopathy

Abstract

Purpose: To test the effects of advanced glycation end products (AGEs), which are increased in vitreous of diabetic patients, on photolysis of hyaluronan. Methods: Pullulan standards were used as molecular weight (MW) markers to obtain a calibration curve. 0.02% hyaluronan solutions were divided into AGE-added and AGE-free samples; each sample was irradiated using a xenon lamp or kept in the dark. Retention time (RT) was measured for each sample using high-performance liquid chromatography. Results:RTs and logarithm of MW of pullulan standards were negatively correlated. In hyaluronan samples exposed to light, RT increased significantly for both AGE-added and AGE-free samples compared with samples kept in the dark. RT in AGE-added samples was greater by 3% than that in AGE-free samples (p = 0.02). Conclusions: Exposure to light decreases MW of hyaluronan; addition of AGEs promotes this change. The photosensitizer activity of AGEs may be associated with accelerated depolymerization of hyaluronan in diabetic patients.

Author Contacts

Tetsuya Sugiyama, MD, PhD
Department of Ophthalmology, Osaka Medical College
2-7 Daigaku-cho, Takatsuki
Osaka, 569-8686 (Japan)
Tel. +81 72 683 1221, Fax +81 72 681 8195, E-Mail opt017@poh.osaka-med.ac.jp

Article Information

Received: January 6, 2004
Accepted after revision: June 25, 2004
Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 1, Number of References : 24

Medline Abstract (ID 15627833)

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