<i>Trichophyton rubrum</i> Isolated from AIDS and Human Immunodeficiency Virus-Infected Patients in São Paulo, Brazil: Antifungal Susceptibility and Extracellular Enzyme Production

Vol. 51, No. 1, 2005

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Microbiology

Trichophyton rubrum Isolated from AIDS and Human Immunodeficiency Virus-Infected Patients in São Paulo, Brazil: Antifungal Susceptibility and Extracellular Enzyme Production
B.C.M. Da Silva^{a, e}, M.E. Auler^a, L.S. Ruiz^a, R.F. Gandra^a, J.I. Dos Santos^b, C.R. Paula^a, M.C.N. Yoshioka^c, L.G.M. Castro^d, R.S. Nunes^d, J.P. Bouchara^e, G. Larcher^e, D. Chabasse^e, W. Gambale^a

^aDepartment of Microbiology, Universidade de São Paulo,
^bDepartment of Clinical Analyses, Universidade Federal de Santa Catarina,
^cCenter for Reference and Training in STD/AIDS, São Paulo, and
^dDivision of Dermatology, Faculdade de Medicina, Hospital das Clínicas, Universidade de São Paulo, Brazil;
^eLaboratory of Parasitology-Mycology, GEIHP - UPRES EA 3142, Centre Hospitalier Universitaire, Angers, France

Address of Corresponding Author

Chemotherapy 2005;51:21-26 (DOI: 10.1159/000084019)

Key Words

Trichophyton rubrum
HIV
AIDS
Antifungals
Extracellular enzymes

Abstract

Background: In order to identify intraspecific variations in Trichophyton rubrum and to correlate them to the immunological status of the host, sixty strains isolated from AIDS, HIV-positive and HIV-negative patients were compared for the production of extracellular enzymes and for their susceptibility to several antifungal drugs. Methods: The isolates were tested for their ability to secrete keratinases, proteinases, phospholipases, lipases and DNases. Likewise, we investigated their susceptibility to amphotericin B, ketoconazole, ciclopiroxolamine, griseofulvin, miconazole and tolnaftate. Results: Variations in the Minimal Inhibitory Concentration (MIC₈₀) values were observed for all antifungals tested, but they were similarly distributed among the three clinical groups. Griseofulvin showed the most prominent differences among the three groups of isolates. Regarding enzyme secretion, all samples secreted keratinases and DNases, while none secreted phospholipases. Proteinases and lipases were secreted by some of them. Conclusions: The differences among isolates of the three groups were not statistically significant and therefore could not be ascribed to a given clinical status. Intraspecific variations similarly occurred in each group, irrespective of the immunological status of the patients.

Author Contacts

Bosco Christiano Maciel Da Silva, MSc
ICB II, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374
Ed. Biomédicas II, Cidade Universitaria
São Paulo, SP CEP 05508-900 (Brasil)
Tel. +55 11 3091 7294, Fax +55 11 3091 7354, E-Mail bosco.christiano@uol.com.br

Article Information

Received: April 14, 2004
Accepted after revision: September 24, 2004
Published online: February 17, 2005
Number of Print Pages : 6
Number of Figures : 1, Number of Tables : 6, Number of References : 26

Medline Abstract (ID 15722629)

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