Experimental Model for Proliferative Vitreoretinopathy by Intravitreal Dispase: Limited by Zonulolysis and Cataract

Vol. 220, No. 4, 2006

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Original Paper

Experimental Model for Proliferative Vitreoretinopathy by Intravitreal Dispase: Limited by Zonulolysis and Cataract
Martina T. Kralinger^{a, b}, Gerhard F. Kieselbach^{a, b}, Monika Voigt^{b, c}, Brandy Hayden^b, Eleut Hernandez^b, Viviana Fernandez^b, Jean-Marie Parel^{b, d, e}

^aDepartment of Ophthalmology, Innsbruck Medical University, Innsbruck, Austria;
^bOphthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Fla., USA;
^cOphthalmic Clinic of Virchow/Charité Hospital, Humboldt University of Berlin, Berlin, Germany;
^dCHU Sart-Tilman, Department of Ophthalmology, University of Liège, Liège, Belgium;
^eUniversity of Paris, Hopital de l'Hotel-Dieu, Paris, France

Address of Corresponding Author

Ophthalmologica 2006;220:211-216 (DOI: 10.1159/000093073)

Key Words

Dispase
Experimental model
Intravitreal injection
Proliferative vitreoretinopathy
Cataract
Zonulolysis

Abstract

Background: The intravitreal injection of dispase has been shown to be a valuable method for induction of experimental PVR. The goal of the present study was to gain additional information about potential side effects associated with this method. Methods: Twenty-one pigmented rabbits received a single injection of dispase under topical anesthesia to one eye only, contralateral eyes served as untreated control. The animals were injected with doses from 0.045 to 0.065 units of dispase: 8 animals received 0.045 units, 9 animals 0.055 units and 4 animals 0.065 units. Results: Proliferative vitreoretinopathy occurred in 81% of the treated eyes. In 90% cataract formation was observed. Lens luxation was present in 47.3% of the cataract eyes. Conclusion: Intravitreal injection of dispase resulted in the reproducible induction of PVR in addition to cataract formation and lens luxation. Whether these effects may all be associated with a toxic reaction or whether the proliferative changes are solely triggered by endogenous reactions similar to the pathomechanism of human PVR and whether the cataract formation and the lens luxation may be avoided by changing the method of injection require further investigation.

Author Contacts

Prof. Dr. Martina Kralinger
Universitätsklinik für Augenheilkunde und Optometrie
Medizin Universität Innsbruck, Anichstrasse 35
AT-6020 Innsbruck (Austria)
Tel. +43 512 504 24184, Fax +43 512 504 23740, E-Mail martina.kralinger@uibk.ac.at

Article Information

Received: June 4, 2004
Accepted after revision: August 29, 2005
Number of Print Pages : 6
Number of Figures : 7, Number of Tables : 1, Number of References : 28

Medline Abstract (ID 16785750)

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