Expression Patterns of <i>Ets2 </i>Protein Correlate with Bone-Specific Proteins in Cell-Seeded Three-Dimensional Bone Constructs

Vol. 186, No. 4, 2007

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Original Paper

Expression Patterns of Ets2 Protein Correlate with Bone-Specific Proteins in Cell-Seeded Three-Dimensional Bone Constructs
Felix Wanschitz, Elisabeth Stein, Walter Sutter, Doris Kneidinger, Konstantin Smolik, Franz Watzinger, Dritan Turhani

Department of Cranio-Maxillofacial and Oral Surgery, General Hospital of Vienna, Vienna, Austria

Address of Corresponding Author

Cells Tissues Organs 2007;186:213-220 (DOI: 10.1159/000107556)

Key Words

Bone tissue engineering
Ets2
Mesenchymal cambial layer precursor cells
Osteoblast
Protein expression
Western blot

Abstract

The transcription factor Ets2 and its transcriptional targets osteopontin (OPN) and osteocalcin (OC) are expressed in tissue-engineered bone constructs in vitro. Up to now little is known about the role of Ets2 in tissue-engineering applications. This study was intended to investigate the hypothesis that protein expression of Ets2 is correlated with the expression of bone-specific proteinsin tissue-engineeredbone constructs. Cell-seeded three-dimensional bone constructs manufactured with osteoblastic cells and poly(lactic-co-glycolic acid) polymer fleeces over a period of 21 days were analyzed by SDS-PAGE and Western blotting. The protein expression of OPN, OC, osteonectin and collagen type I was analyzed. Cellularity, alkaline phosphatase-specific activity and histology confirmed the osteoblastic phenotype of the constructs. Correlations between Ets2 expression and OPN and Ets2 and collagen type I expression could be detected during the phase of late osteoblastic differentiation between days 9 and 21. The correlation between OC and collagen type I was significant in this late stage of osteoblastic differentiation. These results suggest that there is a strong interplay of Ets2 with bone-specific proteins in cell-seeded three-dimensional bone constructs. This study is a crucial step to elucidate the complex interplay of bone-related proteins in the application of bone tissue engineering.

Author Contacts

Dr. Dritan Turhani
Department of Cranio-Maxillofacial and Oral Surgery, General Hospital of Vienna
Währinger Gürtel 18-20, AT-1090 Vienna (Austria)
Tel. +43 1 40 400 4252, Fax +43 1 40 400 4253
E-Mail dritan.turhani@meduniwien.ac.at

Article Information

Accepted after revision: May 8, 2007
Published online: August 16, 2007
Number of Print Pages : 8
Number of Figures : 3, Number of Tables : 0, Number of References : 44

Medline Abstract (ID 17703088)

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