Original Paper

Dietary Supplementation with Lactic Acid Bacteria Attenuates the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice in a Strain-Dependent Manner
Hideyuki Wakabayashi, Chie Nariai, Fumiyo Takemura, Wakako Nakao, Daisuke Fujiwara

Central Laboratories for Frontier Technology, Kirin Brewery Co., Ltd., Yokohama, Japan

Address of Corresponding Author

Int Arch Allergy Immunol 2008;145:141-151 (DOI: 10.1159/000108139)

  Key Words

• Atopic dermatitis
• IgE
• IL-12
• Lactic acid bacteria
• Th1/Th2 balance

  Abstract

Background: Dietary supplementation with lactic acid bacteria (LAB) is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis (AD). However, the influence of different bacterial strains and their immunomodulating capacities is still largely unknown. Methods: AD-like skin lesions were induced by sensitization to and repeated challenges with picrylchloride in the Th2-skewed NC/Nga mouse strain. The effects of LAB supplementation were assessed over time by monitoring clinical scores and plasma IgE levels. In some cases, mast cell infiltration, cutaneous hypersensitivity responses and cytokine mRNA expression in auricles were also examined. Additionally, cytokine production in vitro and cytokine mRNA accumulation in major lymphoid tissues were measured, comparing Lactobacillus paracasei KW3110 with L. rhamnosus GG (LGG). Results: Supplementation with KW3110 significantly reduced the development of AD-like skin lesions, accompanied by less mast cell infiltration and lower plasma IgE levels. KW3110 also suppressed immediate hypersensitivity reactions and IL-4 mRNA expression in the auricles. These preventive effects sustained when supplementation was terminated; moreover, inhibitory effects were also observed even when supplementation was initiated after the onset of symptoms. In accordance with its effects on IL-12 and IL-4 production in vitro, KW3110 prevented the emergence of clinical symptoms more effectively than LGG in vivo. Conclusions: Supplementation with KW3110 significantly attenuated the onset and exacerbation of AD-like symptoms in NC/Nga mice. The effects were more prominent than those obtained with LGG, suggesting the importance of differences between LAB strains and their immunomodulating capacity.

Copyright © 2007 S. Karger AG, Basel

  Author Contacts

Correspondence: Hideyuki Wakabayashi
Central Laboratories for Frontier Technology
Kirin Brewery Co., Ltd., 1-13-5, Fukuura, Kanazawa-ku
Yokohama 236-0004 (Japan)
Tel. +81 45 330 9004, Fax +81 45 788 4047, E-Mail hwakabayashi@kirin.co.jp

  Article Information

Received: December 11, 2006
Accepted after revision: May 30, 2007
Published online: September 11, 2007
Number of Print Pages : 11
Number of Figures : 9, Number of Tables : 0, Number of References : 47