Original Article

Effect of Prolonged c-Kit Receptor Inhibition by Imatinib Mesylate on the Uterine Contractility of Pregnant Rabbits
G. Hutchings^a, T. Gevaert^b, J. Deprest^a, B. Nilius^c, D. De Ridder^b

Departments of
^aObstetrics and Gynaecology,
^bUrology, and
^cPhysiology, University Hospital Gasthuisberg, Leuven, Belgium

Address of Corresponding Author

Gynecol Obstet Invest 2008;65:108-111 (DOI: 10.1159/000109080)

  Key Words

• Imatinib mesylate
• Parturition
• Myometrium
• Myometrial interstitial cells

  Abstract

Background: The c-kit receptor expressed by interstitial cells in the gastrointestinal tract is crucial to their pacemaking function. The function of similar c-kit-expressing myometrial cells is unknown. Methods: Imatinib mesylate, a specific c-kit receptor antagonist, was administered to pregnant New Zealand white rabbits (term = 31 days, n = 35) from day 27 gestation by intramuscular injection twice daily at high (50 µg/kg) or medium (10 µg/kg) dose and compared with a control group injected with vehicle only. In a second phase, two further groups received imatinib at medium or low (1 µg/kg) dose for a longer duration starting from day 18 until delivery. Three does from the latter groups as well as controls underwent myometrial biopsy under general anesthesia after spontaneous vaginal birth. Contractility was recorded by isometric tensiometry. The outcome measures were delay of parturition and in vitro contractility characteristics. Results: High-dose imatinib induced early delivery when compared with the control group (28.6 vs. 30.7 days, p < 0.001). The other groups delivered at term. No effect on in vitro contractility was apparent in any of the groups. Conclusions: c-kit receptor inhibition in pregnant rabbits does not delay significantly the length of gestation or change myometrial contractility in vitro.

Copyright © 2007 S. Karger AG, Basel

  Author Contacts

G. Hutchings
Centre for Surgical Technologies
Minderbroedersstraat 17
BE-3000 Leuven (Belgium)
Tel. +32 479 574 039, Fax +32 1633 7821, E-Mail graham.hutchings@student.kuleuven.be

  Article Information

Received: November 21, 2006
Accepted after revision: April 26, 2007
Published online: October 1, 2007
Number of Print Pages : 4
Number of Figures : 0, Number of Tables : 2, Number of References : 17