Original Paper

Purinergic Receptors and Calcium Signalling in Human Pancreatic Duct Cell Lines
Mette Hansen^*, Simon Krabbe^*, Ivana Novak

Department of Biology, August Krogh Building, University of Copenhagen,
^*contributed equally

Address of Corresponding Author

Cell Physiol Biochem 2008;22:157-168 (DOI: 10.1159/000149793)

  Key Words

• Purinergic receptors
• P2X7 receptor
• P2X4 receptor
• Pancreatic ducts
• ATP release

  Abstract

Purinergic receptors regulate various processes including epithelial transport. There are several studies on P2 receptors in pancreatic ducts of various species, but relatively little is known about these receptors in human tissue. The aim of this study was to identify purinergic receptors in human pancreatic duct cell lines PANC-1 and CFPAC-1. Expression of P2 receptors was examined using RT-PCR and immunocytochemistry. Both cell lines, and also Capan-1 cells, express RNA transcripts for the following receptors: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11-14 and P2X1, P2X2, P2X4, P2X5, P2X6 and P2X7. Using Fura-2 and single-cell imaging we tested effects of various nucleotide analogues on intracellular Ca²⁺ signals in PANC-1 and CFPAC-1 cells. The cell lines responded to all nucleotides with the following efficiency: UTP ATP = ATPS > BzATP. ATP, UTP and ATPS elicited oscillatory responses. BzATP, commonly used to stimulate P2X7 receptors, elicited non-oscillatory and transient Ca²⁺ responses. Ivermectin, a potentiator of P2X4 receptors, increased Ca²⁺ signals evoked by ATP. The single cell Ca²⁺ measurements indicated functional expression of P2Y2 and other P2Y receptors, and notably expression of P2X4 and P2X7 receptors. Expression of P2Y2, P2X4 and P2X7 receptors was confirmed by immunocytochemistry. This fingerprint of P2 receptors in human pancreatic duct models forms the basis for studying effect of nucleotides on ion and fluid secretion, as well as on Ca²⁺ and tissue homeostasis relevant in physiology and pathophysiology of pancreas.

Copyright © 2008 S. Karger AG, Basel

  Author Contacts

Ivana Novak
Department of Biology, August Krogh Building
Universitetsparken 13, University of Copenhagen
DK 2100 Copenhagen (Denmark)
Tel. +45 3532 1645, Fax: +45 3532 1567, E-Mail inovak@bio.ku.dk

  Article Information

Accepted: June 19, 2008
Published online: July 25, 2008
Number of Print Pages : 12