Paper

Chromosome identification in human oocytes and polar bodies by spectral karyotyping
C. Márquez, J. Cohen, S. Munné

Institute for Reproductive Medicine and Science at Saint Barnabas Medical Center, Livingston, NJ (USA)

Address of Corresponding Author

Cytogenet Cell Genet 1998;81:254-258 (DOI: 10.1159/000015040)

  Abstract.

Sixty unfertilized human oocytes and two fresh polar bodies were karyotyped by spectral karyotyping (SKY). The oocytes were provided by 29 women ranging from 30 to 42 yr of age. The mean hybridization efficiency for oocytes was 95.2% (60/63). Nondisjunction of bivalent chromosomes (13.3%) and predivision of sister chromatids at meiosis I (3.3%) were unequivocally determined by analysis first with SKY and then fluorescence in situ hybridization. Four oocytes (6.7%) were hyperhaploid, six (10.0%) were hypohaploid, one (1.7%) showed balanced predivision, and another (1.7%) was diploid. No specific structural rearrangements were detected. This study demonstrates that the SKY technique can be used successfully as an alternative method of karyotyping second meiotic metaphase chromosomes from human oocytes and polar bodies in appropriate spreads.

  Author Contacts

Request reprints from Dr. Carmen Márquez-Guevara, 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052-1023 (USA); telephone: 201-243-1614 ext 33; fax: 201-243-6235; e-mail: carmen.marquez@embryos.net.

  Article Information

Received: Received 24 March 1998;
revision accepted 1 May 1998.
Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 3, Number of References : 30