Comparison of the New OPS Imaging Technique with Intravital Microscopy: Analysis of the Colon Microcirculation

Vol. 34, No. 1-2, 2002

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Surgical Research - A Discipline at the Interface of the Basic Sciences and the Clinic
Festschrift Dedicated to Konrad Meßmer on the Occasion of his 65th Birthday
Editors: A. Baethmann, Munich; G. Enders, Munich; F. Krombach, Munich; N. Plesnila, Munich

Surgical Research - The World of Microcirculation

Comparison of the New OPS Imaging Technique with Intravital Microscopy: Analysis of the Colon Microcirculation
P. Biberthaler^a, S. Langer^b

^aDepartment of Surgery, Klinikum der Ludwig Maximilians Universität München-Innenstadt, Munich, and
^bClinic for Plastic and Hand Surgery/Burn Unit, BG-Hospital Bergmannsheil, Ruhr University, Bochum,Germany

Address of Corresponding Author

Eur Surg Res 2002;34:124-128 (DOI: 10.1159/000048898)

Key Words

Microcirculation
Intravital microscopy
Intestinal
OPS imaging
Inflammatory bowel disease

Abstract

Background: The OPS imaging technique has been introduced for in vivo assessment of microcirculation in humans. The aim of this study was to validate the new technique against intravital fluorescence microscopy (IFM) for the visualization of colon microcirculation in a murine model of inflammatory bowel disease (IBD). Method: IBD was induced in Balb/c mice by dextran sulfate sodium, controls received normal water. In each animal, both the CYTOSCAN^TM A/R and IFM were used to image the microcirculation (n = 7 in each group). The postcapillary venular diameter was analyzed on the colon muscularis and mucosa. Results: The venular diameter correlated significantly between both methods representing the good correspondence between both methods. Conclusion: Our study demonstrates that the new technique for visualization of microcirculation without use of fluorescent dyes, the OPS imaging, allows for quantitative measurement of a key microcirculatory parameters of the mouse colon.

Author Contacts

Dr. Peter Biberthaler, Chirurgische Klinik und Poliklinik
Klinikum der Ludwig-Maximilians-Universität München-Innenstadt
Nussbaumstr. 20, D-80336 München (Germany)
Tel. +49 89 5160 2511, Fax +49 89 5160 4437
E-Mail Peter.Biberthaler@ch.i.med.uni-muenchen.de

Article Information

Number of Print Pages : 5
Number of Figures : 2, Number of Tables : 1, Number of References : 23

Medline Abstract (ID 11867912)

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