International equine gene mapping workshop report: a comprehensive linkage map constructed with data from new markers and by merging four mapping resources

Vol. 111, No. 1, 2005

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Chromosome/Gene Workshops or Reports

International equine gene mapping workshop report: a comprehensive linkage map constructed with data from new markers and by merging four mapping resources
M.C.T. Penedo^a, L.V. Millon^a, D. Bernoco^b, E. Bailey^c, M. Binns^d, G. Cholewinski^e, N. Ellis^f, J. Flynn^g, B. Gralak^h, A. Guthrieⁱ, T. Hasegawa^j, G. Lindgren^k, L.A. Lyons^l, K.H. Røed^m, J.E. Swinburneⁿ, T. Tozaki^o

^aVeterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis, CA (USA);
^bStormont Laboratories, Inc., Woodland, CA (USA);
^cMH Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY (USA);
^dThe Royal Veterinary College, London (UK);
^eHorse Genetic Markers Laboratory, Agricultural University of Poznan (Poland);
^fUniversity of Sydney, Faculty of Veterinary Science, Centre for Advanced Technologies in Animal Genetics and Reproduction-ReproGen, Camden (Australia);
^gWeatherby's Ireland Blood Typing Laboratory, Kildare (Ireland);
^hInstitute of Genetics and Animal Breeding, PAS, Jastrzebiec, Wolka Kosowska (Poland);
ⁱUniversity of Pretoria, Onderstepoort (Republic of South Africa);
^jJapan Racing Association, Utsunomiya (Japan);
^kDepartment of Evolutionary Biology and Department of Medical Biochemistry and Microbiology, UppsalaUniversity(Sweden);
^lDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis,CA(USA);
^mDepartment of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Oslo (Norway);
ⁿAnimal Health Trust, Newmarket (UK);
^oDepartment of Molecular Genetics, Laboratory of Racing Chemistry, Utsunomiya (Japan)

Address of Corresponding Author

Cytogenet Genome Res 2005;111:5-15 (DOI: 10.1159/000085664)

Abstract.

A comprehensive male linkage map was generated by adding 359 new, informative microsatellites to the International Equine Gene Map half-sibling reference families and by combining genotype data from three independent mapping resources: a full sibling family created at the Animal Health Trust in Newmarket, United Kingdom, eight half-sibling families from Sweden and two half-sibling families from the University of California, Davis. Because the combined data were derived primarily from half-sibling families, only autosomal markers were analyzed. The map was constructed from a total of 766 markers distributed on the 31 equine chromosomes. It has a higher marker density than that of previously reported maps, with 626 markers linearly ordered and 140 other markers assigned to a chromosomal region. Fifty-nine markers (7%) failed to meet the criteria for statistical evidence of linkage and remain unassigned. The map spans 3,740 cM with an average distance of 6.3 cM between markers. Fifty-five percent of the intervals are 5 cM and only 3% 20 cM. The present map demonstrates the cohesiveness of the different data sets and provides a single resource for genome scan analyses and integration with the radiation hybrid map.

Author Contacts

Request reprints from M.C.T. Penedo, Veterinary Genetics Laboratory
University of California, One Shields Avenue
Davis, CA 95616-8744 (USA); telephone: 530-752-7460
fax: 530-752-3556; e-mail: mctorrespenedo@ucdavis.edu

Article Information

This work was conducted under the auspices of and with support from The Dorothy Russell Havemeyer Foundation, Inc. Workshop support was also provided by funds available through the USDA-NRSP-8 horse technical committee. Individual laboratories received financial support from many other sources and we gratefully acknowledge the contributions of the American Quarter Horse Foundation, The Morris Animal Foundation, Laboratory of Racing Chemistry and Japan Racing Association, Utsunomiya, Japan, Veterinary Genetics Laboratory at the University of California, Davis, Polish State Committee for Scientific Research (Grant no. 5 P06D 027 18), Horserace Betting Levy Board and the Childwick Trust, United Kingdom, Norwegian Trotting Association, Norway and The Knut and Alice Wallenberg Foundation, Sweden.

Manuscript received 26 August 2004;
accepted in revised form for publication by M. Schmid 26 August 2004.
Number of Print Pages : 11
Number of Figures : 1, Number of Tables : 1, Number of References : 26

Medline Abstract (ID 16093715)

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