Grepafloxacin Concentration in Ocular Tissues after Intravenous Infusion in Rabbits with Intraocular Inflammation

Vol. 37, No. 6, 2005

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Original Paper

Grepafloxacin Concentration in Ocular Tissues after Intravenous Infusion in Rabbits with Intraocular Inflammation
S. Pérez-Oliván^a, I. Pinilla^a, M.A. Bregante^{b, c}, C. Solans^b, O. Ruiz Moreno^a, M.A. Garcia^c, F.M. Honrubia^a

^aDepartment of Ophthalmology, University Hospital Miguel Servet, Zaragoza,
^bDepartment of Analytical Chemistry, and
^cDepartment of Pharmacology and Physiology, Faculty of Veterinary Medicine, University of Zaragoza, Zaragoza, Spain

Address of Corresponding Author

Ophthalmic Res 2005;37:335-340 (DOI: 10.1159/000088262)

Key Words

Fluoroquinolones
Grepafloxacin
Endophthalmitis
Inflammation
Ocular barriers

Abstract

Purpose: To determine the penetration of grepafloxacin into ocular tissues during experimental ocular inflammation. Methods: 10 albino and 10 pigmented rabbits underwent a continuous intravenous infusion of the drug 24 h after injecting Salmonella typhimurium toxin intravitreously, creating ocular inflammation. The animals were killed and grepafloxacin levels were determined in plasma and ocular tissues using high performance liquid chromatography. Results: Grepafloxacin levels achieved a steady-state plasma concentration of 1.5 µg/ml. The drug diffused more towards vascularized tissues (chorioretina and iris) in both albino and pigmented rabbits with a tissue/serum ratio higher than 1. Grepafloxacin showed more affinity to pigmented tissue, rising levels of 40,000-50,000 ng/g in the chorioretina and iris in pigmented animals. After inflammation, grepafloxacin intraocular penetration increased in albino animals with levels exceeding the minimum inhibitory concentration for the most common ocular pathogens. Conclusion: Grepafloxacin intraocular penetration is higher in pigmented tissues. Ocular inflammation increases the drug penetration into the vitreous.

Author Contacts

Dra. S. Pérez-Oliván
Hospital Universitario Miguel Servet
C/Isabel la Catolica 1-3
ES-50009 Zaragoza (Spain)
Tel. +34 976 765558, Fax +34 976 351893, E-Mail sperezo@able.es

Article Information

Received: May 7, 2004
Accepted after revision: November 19, 2004
Published online: September 12, 2005
Number of Print Pages : 6
Number of Figures : 3, Number of Tables : 3, Number of References : 31

Medline Abstract (ID 16158011)

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