Effect of UVA and UVB Irradiation on the Metabolic Profile of Rabbit Cornea and Lens Analysed by HR-MAS <sup>1</sup>H NMR Spectroscopy

Vol. 38, No. 2, 2006

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Original Paper

Effect of UVA and UVB Irradiation on the Metabolic Profile of Rabbit Cornea and Lens Analysed by HR-MAS ¹H NMR Spectroscopy
May-Britt Tessem^a, Anna Midelfart^a, Jitka ejková^c, Tone F. Bathen^b

Departments of
^aNeuroscience, Faculty of Medicine, and
^bCirculation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway;
^cInstitute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Address of Corresponding Author

Ophthalmic Res 2006;38:105-114 (DOI: 10.1159/000090511)

Key Words

Corneal metabolism
Lens metabolism
Metabonomics
Cataract
Ultraviolet radiation
Magic angle spinning

Abstract

Purpose:The aim of the study was to investigate the metabolic profiles of intact rabbit corneas and lenses exposed to UVA and UVB radiation by using high-resolution (HR) magic angle spinning (MAS) ¹H nuclear magnetic resonance (NMR) spectroscopy and pattern recognition methods. Methods:Adult albino rabbits were exposed to UVA (366 nm, 0.589 J/cm²) or UVB (312 nm, 1.667 J/cm²) radiation for 8 min, once a day for 5 days. Three days after the last irradiation day, samples of corneas and lenses were dissected. HR-MAS ¹H NMR spectroscopy combined with pattern recognition methods (principal component analysis and soft independent modelling of class analogy) and one-way ANOVA were applied to obtain metabolic information from intact corneal and lens tissue. Results: UVB irradiation caused statistically significant metabolic changes in the rabbit corneas. A decrease in metabolites as ascorbate (84%), myo-inositol (59%), hypotaurine (91%) and choline (76%) was observed. Exposure to UVA radiation caused no significant metabolic alteration in this tissue. The metabolic profile of the rabbit lenses showed no detectable changes after UVA or UVB exposure. Conclusions:The combination of HR-MAS ¹H NMR spectroscopy and multivariate methods proved effective to analyse intact corneal and lens tissue after exposure to UV radiation of different wavelengths. By avoiding extraction methods and obtaining complete metabolic profiles from one sample, HR-MAS ¹H NMR spectroscopy provided important information about metabolic alteration occurring in rabbit corneal and lens tissue after UV exposure.

Author Contacts

May-Britt Tessem
Department of Neuroscience
Norwegian University of Science and Technology (NTNU), MTFS
NO-7489 Trondheim (Norway)
Tel. +47 73 868 727, E-Mail may-britt.tessem@ntnu.no

Article Information

Received: March 14, 2005
Accepted: September 5, 2005
Published online: December 22, 2005
Number of Print Pages : 10
Number of Figures : 5, Number of Tables : 0, Number of References : 41

Medline Abstract (ID 16374053)

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