Cytogenetic sensitivity of G0 lymphocytes of Fanconi anemia patients and obligate carriers to mitomycin C and ionizing radiation

Vol. 119, No. 3-4, 2007

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Original Article

Cytogenetic sensitivity of G0 lymphocytes of Fanconi anemia patients and obligate carriers to mitomycin C and ionizing radiation
A. Mohseni-Meybodi^a, H. Mozdarani^a, P. Vosough^b

^aDepartment of Medical Genetics, School of Medical Sciences, Tarbiat Modares University,
^bHematology and Oncology Department Iran University of Medical Sciences, Tehran (Iran)

Address of Corresponding Author

Cytogenet Genome Res 2007;119:191-195 (DOI: 10.1159/000112060)

Abstract.

It is well known that Fanconi anemia (FA) patients show a hypersensitivity to the effect of cross-linking agents such as mitomycin C (MMC) and diepoxybutane (DEB), while the sensitivity of these patients to ionizing radiation is still controversial. Fanconi anemia heterozygotes do not show a hypersensitivity to the above mentioned agents compared to normal individuals. To examine the radio-sensitivity of Fanconi anemia patients and heterozygotes, ten patients and 13 heterozygotes were enrolled in this study. Standard metaphase analysis for detection and verification of radio-sensitivity was used to establish the relationship between gamma -ray and chromosome breakages in these groups. Statistical analysis was used for the assessment of aberrations including chromatid and chromosome breaks and exchanges. Results of chromosome aberration yield that: (i) differentiation between obligate carriers and the control group after MMC treatment and gamma irradiation was not possible; (ii) homozygotes were clearly distinguishable from heterozygotes and controls after MMC treatment; (iii) FA patients don't show hypersensitivity to gamma irradiation compared to normal controls and heterozygous carriers.

Author Contacts

Request reprints from Hossein Mozdarani, Ph.D
Department of Medical Genetics, School of Medical Sciences
Tarbiat Modares University, P.O. Box: 14115-111, Tehran (Iran)
telephone +98 21 8801 1001; fax: +98 21 8800 6544
e-mail: mozdarah@modares.ac.ir

Article Information

This work was supported by the Research Department of the Tarbiat Modares University.

Accepted in revised form for publication by M. Schmid,: 8 November 2007.
Published online: February 01, 2008
Number of Print Pages : 5
Number of Figures : 0, Number of Tables : 3, Number of References : 36

Medline Abstract (ID 18253028)

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