Original Paper

Intermolecular Reducible Cross-Links in Rat Glomerular Basement Membrane
Van-Yu Wu, Margo Panush Cohen

University of Medicine and Dentistry of New Jersey, Department of Medicine, Newark, N.J., USA

Address of Corresponding Author

Renal Physiol 1983;6:232-239 (DOI: 10.1159/000172905)

  Key Words

• Basement membrane
• Collagen
• Cross-links

  Abstract

Glomerular basement membrane (GBM) was purified from adult rats and treated with tritiated borohyride for analysis of the reducible cross-links. After acid hydrolysis, samples were subjected, with and without prior gel filtration on Biogel P-2, to a chromatographic system standardized with known cross-links. The major peak of radioactivity co-eluted with authentic di-hydroxylysinonorleucine (di-OHLNL) standard. Derivation of this cross-link from lysine was corroborated by demonstrating its presence in GBM purified after incubation of isolated glomeruli with [¹⁴C]-lysine and after in vivo injection of [¹⁴C]-lysine. GBM labeled in vitro with radioactive lysine also contained peaks co-eluting with hydroxylysinonorleucine and lysinonorleucine standards, as well as at unidentified positions in the chromatogram. The findings indicate that rat GBM contains lysine-derived cross-links, of which di-OHLNL is the major reduced form.

Copyright © 1983 S. Karger AG, Basel

  Author Contacts

Margo P. Cohen, MD, University of Medicine and Dentistry of New Jersey, Department of Medicine, 100 Bergen Street, Newark, NJ 07103 (USA)

  Article Information

Accepted: February 28, 1983
Published online: November 06, 2008
Number of Print Pages : 8