Brain Energy Metabolism: Relationship to Development and Disease
Selected Papers from the 4th International Conference on Brain Energy Metabolism, Eynsham Hall/Oxford (UK), August 14–18, 1999
Guest Editors: John Edmond, Los Angeles, Calif.; John B. Clark, London

MRS and Brain Energy Metabolism

Brain Creatine Kinase and Creatine Transporter Proteins in Normal and Creatine-Treated Rabbit Pups
Téa Kekelidze^a, Igor Khait^a, Anthony Togliatti^a, David Holtzman<sup>a,b

a</sup>Department of Radiology, Brigham and Women’s Hospital and Harvard Medical School, and
^bDepartment of Neurology, Massachusetts General Hospital, Boston, Mass., USA

Address of Corresponding Author

Dev Neurosci 2000;22:437-443 (DOI: 10.1159/000017473)

  Key Words

• Mitochondrial creatine kinase
• Cytosolic creatine kinase
• Brain
• Creatine
• ³¹P NMR spectroscopy

  Abstract

Systemic creatine (Cr) supplementation increases brain phosphocreatine (PCr) and prevents hypoxic seizures in 15-day-old rabbits . Between 5 and 30 days of age during normal development, rabbit gray matter mitochondrial creatine kinase (Mi-CK) increases 400% while cytosolic CK (BB-CK) increases 60%. In white matter, both isoenzymes show smaller, similar increases (40%) during this period. The Cr transporter protein decreases 60% between 5 and 15 days in both regions. In vivo CK rate constants measured by ³¹P nuclear magnetic resonance increase 30% between 10 and 20 days, and then fall 50% between 20 and 30 days in predominantly gray matter slices. Similar maturational changes are seen in predominantly white matter slices. Injecting Cr at 15 days does not significantly change BB-CK or Mi-CK isoenzymes or the in vivo CK reaction rate constants. Thus, the largest change in the CK system associated with suppression of hypoxic seizures in Cr-treated rabbits is increased PCr in gray and white matter.

Copyright © 2000 S. Karger AG, Basel

  Author Contacts

Téa Kekelidze, PhD
Department of Radiology, Brigham and Women’s Hospital
221 Longwood Ave., LMRC Bldg, Boston, MA 02115 (USA)
Tel. +1 617 732 5623, Fax +1 617 278 0610
E-Mail tkeke@bwh.harvard.edu

  Article Information

Received: Received: February 14, 2000
Accepted: June 30, 2000
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 2, Number of References : 36