Differential Changes of CDK Activities in Glomeruli and Tubules during the Active DNA Synthetic Period after Ischemic Injury

Vol. 86, No. 3, 2000

Free Abstract Article (Fulltext) Article (PDF 311 KB)

Original Paper

Differential Changes of CDK Activities in Glomeruli and Tubules during the Active DNA Synthetic Period after Ischemic Injury
Sung Kwang Park, Won Kim, Chang Hwan Lee, Gou Young Koh

Department of Internal Medicine and Institute for Medical Sciences, National Creative Research Initiatives for Cardiac Regeneration, Chonbuk National University Medical School, Chonju, Republic of Korea

Address of Corresponding Author

Nephron 2000;86:306-314 (DOI: 10.1159/000045786)

Key Words

Cyclin
CDK
CKI
Renal ischemia
Renal regeneration

Abstract

The present study was designed to determine the spatial correlation among extent of DNA synthetic activity, expressions of G₁/S phase cyclins, cyclin-dependent kinases (CDKs) and CIP/KIP family of CDK inhibitors (CKIs), and activities of G₁/S phase CDKs in glomeruli and outer medullae of kidneys during the active regeneration period after ischemic injury. DNA synthetic activity was measured using [³H]-thymidine autoradiogram in the kidney sections. Cyclin, CDK, and CKI proteins were determined by Western blot analysis. CDK activities were determined by phosphorylation amount using specific substrate. The protein levels of cyclins (D1, D3, E, A) and activities of CDK4 and CDK2 were increased concomitant with the induction of DNA synthetic activity in outer medullae, but not in glomeruli, in adult kidneys during DNA synthetic period after ischemic injury. The p27^KIP1 protein, but not the p21^CIP1 protein, increased equally in total kidney, glomeruli, and outer medullae after ischemic injury. These results indicate that renal tubules have an active cyclin/CDK system, while glomeruli, do not have a cyclin/CDK system during active regeneration of kidneys after ischemic injury.

Author Contacts

Sung Kwang Park, MD, PhD
Department of Internal Medicine
Chonbuk National University Medical School, San 2-20, Keum-Am-Dong
Chonju 560-180 (Republic of Korea)
Tel. +82 652 250 1683, Fax +82 652 254 1609, E-Mail parksk@moak.chonbuk.ac.kr

Article Information

Received: Accepted: January 11, 2000
Number of Print Pages : 9
Number of Figures : 4, Number of Tables : 0, Number of References : 28

Medline Abstract (ID 11096288)

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