Original Paper

Digital Chemospectrophotographic Identification of Intracellular Hyperlipidemia in Diabetic Endometrial Epithelial Cells: Structural and Metabolic Basis of Organoatrophy
Bryan L. Garris, David R. Garris

Division of Cell Biology and Biophysics, Schools of Medicine and Biological Sciences, University of Missouri-Kansas City, Kansas City, Mo., USA

Address of Corresponding Author

Pathobiology 2004;71:77-83 (DOI: 10.1159/000074420)

  Key Words

• Diabetes
• Endometrial cytolipidemia
• Triglycerides and free fatty acids
• Digital photochemical microscopy
• Cellular atrophy and involution

  Abstract

Objective: Digital chemospectrophotographic (DCSP) microscopic analysis and evaluation methodology applicable for enhanced cytopathological analysis of diabetes-induced, cytohyperlipidemia-associated cellular involution in endometrial epithelial and stromal tissues that promotes reproductive dysfunction and organoatrophy. Methods: Combined light microscopy (LM), transmission electron microscopy (TEM) and described DCSP evaluation of endometrial samples collected from control (+/?) and genetically diabetic (db/db) C57BL/KsJ, hyperglycemic-hyperinsulinemic (type II) mice, designed to enhance the intracellular localization of chemically specified triglyceride and free fatty acid depositions, on progressive reproductive tract atrophy and cellular involution indices. Results: Compared to both the LM and TEM analysis of cytopathological changes associated with diabetes-induced endometrial involution and reproductive dysfunction, the application of DCSP provided enhanced pathovisual analysis of chemical-specific metabolic alterations and cytoplasmic structural changes which accompany cytohyperlipidemia-induced endometrial epithelial cell apoptosis and reproductive tract atrophy. Conclusions: DCSP analysis provides an enhanced analytical method for the evaluation of cytoplasmic changes associated with the expression of genomic-, endocrine- or metabolic-based disease states by providing intercytoplasmic specific chemical or metabolic substrate alterations to be identified from conventional pathocellular preparations without requiring the use of exogenous ligand binding or fluorescent methodologies, allowing for a more complete metabolic and cellular evaluation of cytoplasmic indices associated with organoatrophy.

Copyright © 2004 S. Karger AG, Basel

  Author Contacts

Dr. David R. Garris
Cell Biology and Biophysics, School of Biological Sciences
University of Missouri-Kansas City, 5007 Rockhill Road
Kansas City, MO 64110 (USA)
Tel. +1 816 235 5857, Fax +1 816 235 6563, E-Mail garrisd@umkc.edu

  Article Information

Received: July 14, 2002
Accepted: May 13, 2003
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 1, Number of References : 14