Association of Single Nucleotide Polymorphisms in the Eosinophil Peroxidase Gene with Japanese Cedar Pollinosis

Vol. 135, No. 1, 2004

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Association of Single Nucleotide Polymorphisms in the Eosinophil Peroxidase Gene with Japanese Cedar Pollinosis
Hiroyuki Nakamura^a, Fumiko Higashikawa^b, Kiyoshi Miyagawa^c, Yoshitaka Nobukuni^c, Tomohiko Endo^d, Toru Imai^d, Kotaro Ozasa^e, Yutaka Motohashi^f, Ichiyo Matsuzaki^g, Shinichiro Sasahara^g, Kotaro Hatta^h, Keiki Oginoⁱ, Akira Eboshida^b

^aDepartment of Environmental Medicine, Kochi University School of Medicine, Nankoku;
^bDepartment of Public Health, Graduate School of Medical and Pharmaceutical Sciences,
^cDepartment of Molecular Pathology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima;
^dDepartment of Otorhinolaryngology, Jikei University School of Medicine, Jikei;
^eDepartment of Social Medicine and Cultural Sciences, Research Institute for Neurological Diseases and Geriatrics, Kyoto Prefectural University of Medicine, Kyoto;
^fDepartment of Public Health, Akita University School of Medicine, Akita;
^gInstitute of Community Medicine, University of Tsukuba, Tsukuba;
^hDepartment of Psychiatry, Juntendo University School of Medicine, Tokyo;
ⁱDepartment of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

Address of Corresponding Author

Int Arch Allergy Immunol 2004;135:40-43 (DOI: 10.1159/000080222)

Key Words

Cedar pollinosis
Eosinophil peroxidase
Polymorphisms
Transmission disequilibrium test

Abstract

Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

Author Contacts

Correspondence to: Dr. Hiroyuki Nakamura
Department of Environmental Medicine, Kochi University School of Medicine
Kohasu, Okoh-cho
Nankoku 783-8505 (Japan)
Tel./Fax +81 88880 2405, E-Mail hiro-n@po.incl.ne.jp

Article Information

Received: September 24, 2003
Accepted after revision: June 1, 2004
Published online: August 13, 2004
Number of Print Pages : 4
Number of Figures : 0, Number of Tables : 1, Number of References : 17

Medline Abstract (ID 15316147)

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