Original Paper

Induction of Apoptosis in HaCaT Cells by Photodynamic Therapy with Chlorin e6 or Pheophorbide a
A. Radestock^a, P. Elsner^a, B. Gitter^b, U.-C. Hipler<sup>a

a</sup>Department of Dermatology and Allergology, Friedrich Schiller University Jena, and
^bBiolitec AG, Jena, Germany

Address of Corresponding Author

Skin Pharmacol Physiol 2007;20:3-9 (DOI: 10.1159/000096166)

  Key Words

• Photodynamic therapy
• Photosensitizers
• Apoptosis
• Caspases
• HaCaT keratinocytes

  Abstract

The two photosensitizers, chlorin e6 and pheophorbide a, were tested in an in vitro model of topical photodynamic therapy (PDT). Both dyes accumulate in HaCaT keratinocytes as verified by fluorescence measurement but pheophorbide a is enriched fivefold more strongly than chlorin e6 after 24 h. HaCaT cells are susceptible to PDT with both dyes. The phototoxicity measured by ATP bioluminescence is caused by necrosis and apoptosis depending on the photosensitizer used and the treatment modality. Chlorin e6 shows higher toxic potential because it elicits nearly 90% cell mortality 24 h after PDT comparable to pheophorbide a but with a fivefold lower rate of accumulation. These results implicate caution with topical PDT of oncologic diseases due to the risk of serious side effects on healthy skin in the course of topical photodynamic treatment. But the lack of dark toxicity and the time-dependent enrichment of both dyes in HaCaT cells are arguments for the application of these sensitizers in topical PDT of non-malign skin disorders. Further studies are necessary to discover appropriate lower doses and mechanisms of action of topical PDT with both compounds.

Copyright © 2007 S. Karger AG, Basel

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  Author Contacts

Dr. Uta-Christina Hipler
Department of Dermatology and Allergology, Friedrich Schiller University Jena
Erfurter Strasse 35
DE-07740 Jena (Germany)
Tel. +49 3641 937 355, Fax +49 3641 937 437, E-Mail Christina.Hipler@med.uni-jena.de

  Article Information

Supported by Biolitec AG and the BMFT project BEO31/0312556.

Received: May 13, 2005
Accepted after revision: February 2, 2006
Published online: October 11, 2006
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 0, Number of References : 32

  Publication Details

Skin Pharmacology and Physiology (Journal of Pharmacological and Biophysical Research)

Vol. 20, No. 1, Year 2007 (Cover Date: December 2006)

Journal Editor: Lademann, J. (Berlin)
ISSN: 1660-5527 (print), 1660-5535 (Online)

For additional information: http://www.karger.com/SPP

  Drug Dosage / Copyright

Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.