Distribution of F-bodies, heterochromatin, and nucleolar organizers in the genome of the central mudminnow, <i>Umbra limi</i>

Vol. 18, No. 4, 1977

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Paper

Distribution of F-bodies, heterochromatin, and nucleolar organizers in the genome of the central mudminnow, Umbra limi
A.D. Kligerman, S.E. Bloom

Department of Poultry Science Cornell University Ithaca N.Y.

Address of Corresponding Author

Cytogenet Cell Genet 1977;18:182-196 (DOI: 10.1159/000130762)

Abstract

Due to the very limited amount of knowledge available on the cytochemistry and architecture of fish chromosomes, an extensive banding study was carried out on chromosomes of the central mudminnow, Umbra limi. Through the use of fluorescent staining, C-banding, and silver staining, the chromosomes were characterized, and an idiogram was constructed showing the location of C-band heterochromatin, F-bodies, and the nucleolar-organizer regions. G-banding was attempted but was unsuccessful. Results of fluorescent staining with 33258 Hoechst and quinacrine HCl raised some interesting questions concerning the cytochemistry of U. limi chromosomes, as well as the specificity of these stains.

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Request reprints from: Mr. Andrew D. Kligerman, Department of Poultry Science, Cornell University, Ithaca, NY 14853 (USA).

Article Information

Manuscript received 3 January 1977
Published online: May 02, 2008
Number of Print Pages : 15

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