ISOBM TD-4 International Workshop on Monoclonal Antibodies against MUC1
Guest Editors: P.D. Rye, Oslo; M.R. Price, Nottingham
TD-4 Workshop Chairmen: J. Hilgers, Amsterdam; K. Nustad, Oslo
San Diego, Calif., November 17-23, 1996

TD-4 Workshop Group 13

Reactivity of Antibodies with Highly Glycosylated MUC1 Mucins from Colon Carcinoma Cells and Bile
Rein Sikut, Anu Sikut, Ke Zhang, Dan Baeckström, Gunnar C. Hansson

Department of Medical Biochemistry, Göteborg University, Gothenburg, Sweden

Address of Corresponding Author

Tumor Biol 1998;19:122-126 (DOI: 10.1159/000056513)

  Key Words

• MUC1
• Colon carcinoma
• Bile

  Abstract

The 55 antibodies submitted to the ISOBM TD-4 Workshop were analysed for their reactivity with core proteins of the heavily glycosylated MUC1 mucins from the colon carcinoma cell line COLO205 and bile. Both these mucins (designated as H-CanAg and SBG2) are highly glycosylated having 15 and 50 sugar residues per oligosaccharide, respectively. Only a few of the antibodies (129, 139, 153 and 162) reacted with both SBG2 and H-CanAg, and not with a control mucin (L-CanAg) having a similar glycosylation as H-CanAg. These antibodies were tested for their ability to catch soluble mucins, and the antibody 162 was found to be good also in this type of assay. The antibodies selected here should be useful for the detection of high glycosylated forms of the MUC1 mucin in tissues and serum.

  Author Contacts

Gunnar C. Hansson
Department of Medical Biochemistry, Göteborg University
Medicinaregatan 9A, S-413 90 Gothenburg (Sweden)
Tel. +46 31 7733488, Fax +46 31 416108
E-Mail gunnar.hansson@medkem.gu.se

  Article Information

Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 1, Number of References : 7