Address of Corresponding Author

J Mol Microbiol Biotechnol 2004;8:104-110 (DOI: 10.1159/000084565)

  Key Words

• Tetracycline repressor
• Operator specificity
• Effector specificity
• Reverse TetR
• Combination

  Abstract

Tet repressor mutants with a shifted effector specificity, preference for a mutant operator sequence or reversion of activity were combined to construct variants bearing two or three phenotypic alterations. TetR alleles with combinations of altered operator and effector specificities can be created by merging the respective residues in a single polypeptide. The mutations giving rise to revTetR, on the other hand, show drastic influences on the ligand binding phenotypes when combined with respective alterations. One TetR variant displays all three phenotypic alterations and thus demonstrates the general possibility of implementing them in one protein.

Copyright © 2004 S. Karger AG, Basel

  Author Contacts

Wolfgang Hillen
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik
Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5
DE-91058 Erlangen (Germany)
Tel. +49 9131 8528081, Fax +49 9131 8528082, E-Mail whillen@biologie.uni-erlangen.de

  Article Information

Abbreviations

TetR = Tetracycline repressor; revTetR = reverse TetR; tc = tetracycline; atc = anhydrotetracycline; 4-ddma-atc = 4-de-dimethylamino-atc; -gal = -galactosidase.

Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 2, Number of References : 21