The Complete Genome Sequence of <i> Bacillus licheniformis </i>DSM13, an Organism with Great Industrial Potential

Vol. 7, No. 4, 2004

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Research Article

The Complete Genome Sequence of Bacillus licheniformis DSM13, an Organism with Great Industrial Potential
Birgit Veith^a, Christina Herzberg^a, Silke Steckel^a, Jörg Feesche^b, Karl Heinz Maurer^b, Petra Ehrenreich^a, Sebastian Bäumer^a, Anke Henne^a, Heiko Liesegang^a, Rainer Merkl^a, Armin Ehrenreich^a, Gerhard Gottschalk^a

^aGöttingen Genomics Laboratory and Competence Centre for Genome Research on Bacteria, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, and
^bHenkel KGaA, VBT Enzymtechnologie, Düsseldorf, Germany

Address of Corresponding Author

J Mol Microbiol Biotechnol 2004;7:204-211 (DOI: 10.1159/000079829)

Key Words

Bacillus licheniformis DSM13
Genome sequence
Bacillus subtilis
Industrial enzymes
Industrial fermentation

Abstract

The genome of Bacillus licheniformis DSM13 consists of a single chromosome that has a size of 4,222,748 base pairs. The average G+C ratio is 46.2%. 4,286 open reading frames, 72 tRNA genes, 7 rRNA operons and 20 transposase genes were identified. The genome shows a marked co-linearity with Bacillus subtilis but contains defined inserted regions that can be identified at the sequence as well as at the functional level. B. licheniformis DSM13 has a well-conserved secretory system, no polyketide biosynthesis, but is able to form the lipopeptide lichenysin. From the further analysis of the genome sequence, we identified conserved regulatory DNA motives, the occurrence of the glyoxylate bypass and the presence of anaerobic ribonucleotide reductase explaining that B. licheniformis is able to grow on acetate and 2,3-butanediol as well as anaerobically on glucose. Many new genes of potential interest for biotechnological applications were found in B. licheniformis; candidates include proteases, pectate lyases, lipases and various polysaccharide degrading enzymes.

Author Contacts

Armin Ehrenreich
Genomics Laboratory and Competence Centre for Genome Research on Bacteria
Institute of Microbiology and Genetics, University of Göttingen
Grisebachstrasse 8, DE–37077 Göttingen (Germany)
Tel. +49 551 393833, Fax +49 551 393793, E-Mail aehrenr@gwdg.de

Article Information

Number of Print Pages : 8
Number of Figures : 4, Number of Tables : 5, Number of References : 53

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