<i>Isodon japonicus</i> Decreases Immediate-Type Allergic Reaction and Tumor Necrosis Factor-α Production

Vol. 135, No. 1, 2004

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Original Paper

Isodon japonicus Decreases Immediate-Type Allergic Reaction and Tumor Necrosis Factor- Production
Tae-Yong Shin^a, Sang-Hyun Kim^b, Cheol-Hee Choi^b, Hye-Young Shin^c, Hyung-Min Kim^d

^aCollege of Pharmacy, Woosuk University, Jeonju,
^bResearch Center for Resistant Cells, College of Medicine, Chosun University, Gwangju,
^cCollege of Pharmacy, Wonkwang University, Iksan,
^dCollege of Oriental Medicine, Kyung Hee University, Seoul, South Korea

Address of Corresponding Author

Int Arch Allergy Immunol 2004;135:17-23 (DOI: 10.1159/000080038)

Key Words

Isodon japonicus
Allergic reaction
Compound 48/80
Anti-dinitrophenyl IgE
Tumor necrosis factor-
Mast cells

Abstract

Background: The immediate-type allergic reaction is involved in many allergic diseases such as asthma, allergic rhinitis and sinusitis. The discovery of drugs for the treatment of immediate-type allergic disease is a very important subject in human health. Isodon japonicus Hara (Labiatae) (IJAE) has been used for centuries as a traditional medicine in Korea and is known to have an anti-inflammatory effect. However, its specific mechanism of action is still unknown. In this report, we investigated the effect of IJAE on the immediate-type allergic reaction and studied its possible mechanisms of action, focusing on the mast cell-mediated allergic reaction. Methods: IJAE extracts were anally administered to mice for high and fast absorption. Compound 48/80-induced mortality and compound 48/80- or immunoglobulin E (IgE)-induced histamine release were measured to evaluate the antiallergic effects of IJAE. The effect of IJAE on the model of local allergic reaction in vivo, passive cutaneous anaphylaxis (PCA), was investigated. The production of tumor necrosis factor- alpha (TNF- alpha ) was measured by Western blotting. Results: IJAE inhibited compound 48/80-induced systemic reactions and plasma histamine release in mice. IJAE decreased the PCA reaction activated by anti-dinitrophenyl (DNP) IgE antibody. IJAE dose-dependently reduced histamine release from rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. Furthermore, IJAE decreased the production of TNF- alpha in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated human mast cells. Conclusion: Our findings provide evidence that IJAE inhibits mast cell-derived immediate-type allergic reactions, and also demonstrate the involvement of TNF- alpha in these effects. We propose the clinical use of IJAE in mast cell-mediated immediate-type allergic diseases.

Author Contacts

Correspondence to: Tae-Yong Shin
College of Pharmacy, Woosuk University
Jeonju, Jeonbuk, 565-701 (South Korea)
Tel. +82 63 290 1572, Fax +82 63 290 1567
E-Mail tyshin@woosuk.ac.kr

Article Information

Received: March 30, 2004
Accepted after revision: May 19, 2004
Published online: July 29, 2004
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 2, Number of References : 36

Medline Abstract (ID 15286441)

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