Characterization of Glucose Uptake by Cultured Rat Podocytes

Vol. 28, No. 1, 2005

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Original Paper

Characterization of Glucose Uptake by Cultured Rat Podocytes
Barbara Lewko^{a, c}, Ewa Bryl^b, Jacek M. Witkowski^b, Elbieta Latawiec^a, Magdalena Goos^a, Nicole Endlich^d, Brunhilde Hähnel^d, Claudia Koksch^d, Stefan Angielski^{a, c}, Wilhelm Kriz^d, Jan Stepinski^{a, c}

Departments of
^aImmunopathology and
^bPathophysiology, Medical University of Gdansk, Gdansk;
^cLaboratory of Cellular and Molecular Nephrology, Medical Research Centre of the Polish Academy of Sciences, Warsaw/Gdansk, Poland, and
^dInstitute for Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany

Address of Corresponding Author

Kidney Blood Press Res 2005;28:1-7 (DOI: 10.1159/000080889)

Key Words

Cultured podocytes
GLUT2
GLUT4
SGLT
Glucose transport

Abstract

The nonmetabolizable glucose analogue [³H]-2-deoxy-D-glucose (³H-2DG) was used to study glucose transport in cultured rat podocytes. Intracellular accumulation of ³H-2DG was linear up to 20 min and was inhibited by cytochalasin B (80% inhibition) and by phlorizin (20% inhibition). Pretreatment with insulin stimulated the ³H-2DG uptake 1.5-fold. A Hill analysis of the rate of glucose transport yielded a V_max value of approximately 10 mM and S_0.5of 7.8 mM. The value h = 1.0 for a Hill coefficient confirmed that glucose uptake exhibited a Michaelis-Menten kinetics. Transporters GLUT2 and GLUT4 were expressed in over 90% podocytes. Of the GLUT2- and GLUT4-expressing cells, approximately one-fourth expressed the membrane-bound fraction. We conclude that cultured rat podocytes possess a differentiated glucose transport system consisting chiefly of facilitative GLUT2 and GLUT4 transporters. It seems likely that a sodium-dependent glucose cotransporter may also be present in these cells.

Author Contacts

Barbara Lewko, PhD
Department of Immunopathology
Medical University of Gdansk
ul. Debinki 7, PL-80-952 Gdansk (Poland)
Tel./Fax +48 58 3492191, E-Mail blew@amedec.amg.gda.pl

Article Information

Accepted: April 29, 2004
Published online: September 20, 2004
Number of Print Pages : 7
Number of Figures : 5, Number of Tables : 1, Number of References : 24

Medline Abstract (ID 15383720)

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