Address of Corresponding Author

Nephron Physiol 2005;99:p85-p89 (DOI: 10.1159/000083764)

  Key Words

• Microarray
• Pathology
• Human diseases

  Abstract

Until recently, diagnostic and prognostic assessment of diseased tissues in a Pathology laboratory relied on histological and immunohistological studies. DNA microarray technology now allows the simultaneous analysis of up to thousands of different genes in histological or cytological specimens. Thus, the microarray techniques offer opportunities for the pathologist to obtain 'molecular signatures' of the state of activity of diseased cells in patient tissue samples, providing new information, such as the biological staging of tumors, a risk assessment of pre-malignant lesions, resistance to, and side effects of, treatment, molecular mechanisms in neurodegeneration and inflammation, and detection of micro-organisms. However, despite the great promise of this revolutionary technology, there are several issues that may undermine the power of the DNA microarray approach. After a short summary of the different forms of microarray and the contribution of this technology to understanding human disease, this short review focuses on the potential pitfalls and the important issues that must be considered when using a DNA microarray. Although this new approach shows great potential, the successful application of gene arrays to diagnostic and prognostic problems requires thoughtful interpretation and a strong correlation with other data, such as clinical, histopathological and immunohistochemical findings.

Copyright © 2005 S. Karger AG, Basel

  Author Contacts

Prof. Paul Hofman
INSERM 0215
Faculty of Medicine
F-06107 Nice Cedex 01 (France)
E-Mail hofman@unice.fr

  Article Information

Published online: February 7, 2005
Number of Print Pages : 5
Number of Figures : 0, Number of Tables : 1, Number of References : 16