Originalarbeit · Original Article

Anti-Inflammatory Mechanisms of the Tibetan Herbal Preparation Padma 28 in the Vessel Wall
M. Exner^a; M. Raith^a; G. Holzer^a; B. Gmeiner^b; O. Wagner^a; S. Kapiotis^a

^aKlinisches Institut für Medizinische und Chemische Labordiagnostik, ^bInstitut für Medizinische Chemie, Medizinische Universität Wien, Austria

Forsch Komplementmed 2006;13(suppl 1):13-17
(DOI: 10.1159/000090435)

  Summary

Background: The Tibetan herbal preparation Padma 28 has been shown to act as an anti-atherosclerotic agent in advanced peripheral arterial occlusive disease. We tested the effect of aqueous Padma 28 extracts on both the Creactive protein (CRP) induced expression of the pro-inflammatory cell adhesion molecule E-selectin and the anti-atherosclerotic protective enzyme heme oxygenase- 1 (HO-1) in human aortic endothelial cells. Methods and Results: According to FACS analysis, quantitative RT-PCR and Western blot, CRP-induced E-selectin expression was completely prevented by aqueous Padma 28 extracts. Additionally, Padma 28 mediated an up to 60-fold upregulation of HO-1 mRNA as measured by quantitative RT-PCR. This upregulation could also be demonstrated on the protein level. Conclusion: Aqueous extracts of the Tibetan herbal preparation Padma 28 inhibit CRP-induced expression of the inflammatory cell adhesion molecule E-selectin and lead to upregulation of the vascular protective enzyme HO-1 in human aortic endothelial cells. These properties may be responsible for its antiatherosclerotic effects in peripheral arterial occlusive disease.

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