Gene Expression

Global gene expression profile after Salmonella enterica Serovar enteritidis challenge in two F8 advanced intercross chicken lines
H. Zhou^{a, b}, S.J. Lamont<sup>a

a</sup>Department of Animal Science, Iowa State University, Ames, IA
^bDepartment of Poultry Science, Texas A&M University, College Station, TX (USA)

Address of Corresponding Author

Cytogenet Genome Res 2007;117:131-138 (DOI: 10.1159/000103173)

  Abstract.

A chicken 13K cDNA microarray was used to profile global gene expression after Salmonella enteritidis (SE) challenge of young chickens. Two F8 advanced intercross lines (AIL), broiler by Leghorn, and broiler by Fayoumi, were studied. Day-old chicks were orally inoculated with SE, and spleens were harvested at day 7 or 8 post-inoculation. The SE bacteria burden in the spleen was quantified. The 20% high and 20% low SE burden birds within each AIL and harvest time were studied by microarray. The loop design was used for pair-comparison between high and low SE burden challenged birds and unchallenged birds, within each AIL and harvest time. The signal intensity of each gene was globally normalized and expressed on the natural log scale. A mixed model including line, treatment, time, array (random effect), dye, and all two-way interactions among treatment, time, and line was used to identify differentially expressed candidate genes at the 1% significance level. The results suggest that genetics, time, and interaction between genetics and time play important roles in gene regulation of SE infection and colonization in chickens. The differentially expressed genes identified in the current study are candidates for detailed hypothesis-driven investigation of genes determining resistance to SE in chickens.

Copyright © 2007 S. Karger AG, Basel

  Author Contacts

Request reprints from Susan J. Lamont
2255 Kildee Hall, Department of Animal Science, Iowa State University
Ames, IA 50011 (USA)
telephone: +1 515 294 4100; fax: +1 515 294 2401
e-mail: sjlamont@iastate.edu

  Article Information

This is a report of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA 50011. This work was supported by Hatch and State of Iowa funds, National Research Initiative Grant No. 2004-35205-14234 from USDA Cooperative State Research, Education, and Extension Service, and Research Grant US-3408-03 from BARD, the Binational Agriculture Research and Development fund.

Manuscript received: 25 June 2006
Accepted in revised form for publication by J. Smith,: 9 August 2006.
Number of Print Pages : 8
Number of Figures : 3, Number of Tables : 5, Number of References : 33