Implication of Dipeptidylpeptidase IV Activity in Human Bronchial Inflammation and in Bronchoconstriction Evaluated in Anesthetized Rabbits

Vol. 75, No. 1, 2008

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Basic Science Investigations

Implication of Dipeptidylpeptidase IV Activity in Human Bronchial Inflammation and in Bronchoconstriction Evaluated in Anesthetized Rabbits
B.N. Landis^a, E. Grouzmann^e, M. Monod^f, N. Busso^g, F. Petak^h, A. Spiliopoulos^b, J.H. Robert^b, I. Szalay-Quinodoz^c, D.R. Morel^d, J.S. Lacroix^a

^aRhinology-Olfactology Unit, Department of Otolaryngology, and Departments of
^bThoracic Surgery,
^cPathology and
^dAnesthesiology, University Hospital of Geneva, Geneva, and
^eDivision de Pharmacologie et Toxicologie Cliniques, Centre Hospitalier Universitaire Vaudois,
^fService de Dermatologie et
^gService de Rhumatologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland;
^hDepartment of Medical Informatics and Engineering, University of Szeged, Szeged, Hungary

Address of Corresponding Author

Respiration 2008;75:89-97 (DOI: 10.1159/000106267)

Key Words

Bronchial mucosa
Bronchoconstriction
Dipeptidylpeptidase IV
Histamine
Inflammation
Nasal mucosa
Upper and lower airways

Abstract

Background: Decreased dipeptidylpeptidase IV (DPPIV) activity within the human nasal mucosa has previously been shown to contribute to the severity of chronic inflammatory rhinosinusitis. Objective: To investigate and correlate the role of DPPIV activity with regard to bronchial inflammation. Methods: DPPIV/CD26 activity/concentration was investigated in the bronchial tissue of human subjects suffering from chronic bronchial inflammation. In addition, the effect of a recombinant Aspergillus fumigatus DPPIV (fuDPPIV) was investigated on histamine-induced bronchoconstriction in anesthetized rabbits. Results and Conclusions: DPPIV/CD26 was present in submucosal seromucous glands, in leukocytes and to a very low degree in endothelial cells of human bronchi. DPPIV activity was correlated with tissue CD26 content measured by immunoassay. As previously reported for the nasal mucosa, DPPIV/CD26 activity was inversely correlated with the degree of airway inflammation. Systemic pretreatment with recombinant fuDPPIV markedly reduced the increase in histamine-induced airway resistance in rabbits. In conclusion, DPPIV activity modulates lower airway tone by degrading unknown peptidic substrates released by histamine in response to an allergen. Contrasting with our observations in the nose, this modulation is apparently not mediated via a neurokinin (NK1) receptor.

Author Contacts

Basile N. Landis, MD
Unité de Rhinologie-Olfactologie, Service d'ORL et de Chirurgie cervico-faciale
Hôpitaux Universitaires de Genève, Rue Micheli-du-Crest 24
CH-1211 Geneva (Switzerland)
Tel. +41 22 372 82 62, Fax +41 22 372 82 40, E-Mail Basile.Landis@hcuge.ch

Article Information

Received: November 30, 2006
Accepted after revision: March 1, 2007
Published online: July 18, 2007
Number of Print Pages : 9
Number of Figures : 5, Number of Tables : 1, Number of References : 21

Medline Abstract (ID 17637510)

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