Histamine Stimulates Interleukin-6 Production through Histamine H1 Receptors in Human Amnion Cells

Vol. 69, No. 1, 2010

Article (References) Article (PDF 363 KB)

Original Article

Histamine Stimulates Interleukin-6 Production through Histamine H1 Receptors in Human Amnion Cells
Akiko Morimitsu^a, Seiji Sumigama^a, Tomomi Kotani^a, Yukio Mano^a, Kanako Suzuki^b, Masako Araki-Taguchi^c, Eiko Yamamoto^a, Hiromi Hayakawa^a, Mayumi Okada^d, Atsuo Itakura^e, Fumitaka Kikkawa^a

^aDepartment of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine,
^bDepartment of Obstetrics and Gynecology, Ogaki Municipal Hospital, Ogaki,
^cDepartment of Obstetrics and Gynecology, International Holy Spirit Hospital, Nagoya,
^dDepartment of Obstetrics and Gynecology, Toyohashi Municipal Hospital, Toyohashi, and
^eDepartment of Obstetrics and Gynaecology, Saitama Medical University, Faculty of Medicine, Saitama, Japan

Address of Corresponding Author

Gynecol Obstet Invest 2010;69:67-72 (DOI: 10.1159/000257663)

Key Words

Histamine
Interleukin-6
Amnion
Allergy
Preterm labor/delivery

Abstract

Background/Aims: Previous studies have stated that maternal allergic diseases are associated with increased risk of preterm labor/delivery, but the underlying mechanisms remain unclear. This study tested the hypothesis that histamine induces interleukin (IL)-6 production in amnion cells. Methods: Using cultured human amnion cells, we examined expression of histamine receptors and effects of histamine on IL-6 production. Results: Reverse transcription-polymerase chain reaction and Western blotting revealed expression of histamine H1 receptor (H1R) and H2 receptor (H2R) in human amnion. Histamine stimulation significantly increased concentrations of IL-6 in conditioned medium, as did tumor necrosis factor-α and IL-1β in positive controls. In addition, the H1R antagonist olopatadine significantly blocked histamine-induced production of IL-6, whereas the H2R antagonist ranitidine did not. Conclusion: Histamine appears to induce IL-6 production through H1R in human amnion cells.

Author Contacts

Seiji Sumigama
Department of Obstetrics and Gynecology
Nagoya University Graduate School of Medicine
Tsurumai-cho 65, Showa-ku, Nagoya 466-8550 (Japan)
Tel. +81 52 744 2261, Fax +81 52 744 2268, E-Mail sumigama@med.nagoya-u.ac.jp

Article Information

Received: April 21, 2009
Accepted after revision: July 9, 2009
Published online: November 12, 2009
Number of Print Pages : 6
Number of Figures : 4, Number of Tables : 0, Number of References : 36

Cited in Web of Science®

Recommend this

This journal is part of the 5th subject package of the Karger

Journal Archive Collection

Information on packages (PDF)
Free sample issues

For non-native English speakers and international authors who would like assistance with their writing before submission, we suggest American Journal Experts for their scientific editing service.