Original Report: Patient-Oriented, Translational Research

KLOTHO Gene Polymorphisms G-395A and C1818T Are Associated with Low-Density Lipoprotein Cholesterol and Uric Acid in Japanese Hemodialysis Patients
Yasuhiko Shimoyama^a, Kentaro Taki^a, Yoko Mitsuda^b, Yoshinari Tsuruta^c, Nobuyuki Hamajima^b, Toshimitsu Niwa^a

Departments of
^aAdvanced Medicine for Uremia, Nagoya University Graduate School of Medicine, and
^bPreventive Medicine/Biostatistics and Medical Decision Making, Nagoya University Graduate School of Medicine, Nagoya,
^c Meiyo Clinic, Toyohashi, Aichi, Japan

Address of Corresponding Author

Am J Nephrol 2009;30:383-388 (DOI: 10.1159/000235686)

  Key Words

• KLOTHO
• Dialysis
• Low-density lipoprotein cholesterol
• Uric acid

  Abstract

Background/Aims: This study aimed to investigate the association of the KLOTHO gene single nucleotide polymorphisms (SNPs), G-395A and C1818T, with various laboratory data in 219 Japanese hemodialysis (HD) patients. Methods: The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay. Results: In HD patients, the allele frequencies of G-395A were 0.847 for the G allele and 0.153 for the A allele and those of the C1818T were 0.829 for the C allele and 0.171 for the T allele. There were no significant differences in allele frequencies of G-395A and those of the C1818T between HD patients and healthy subjects. Multivariate analysis adjusted for age and duration on HD demonstrated that uric acid was significantly high in A allele carriers of G-395A compared with GG genotype in all and female patients. Low-density lipoprotein cholesterol was significantly low in T allele carriers of C1818T compared with CC genotype in all and male patients. Conclusion:KLOTHO gene SNPs G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in HD patients.

Copyright © 2009 S. Karger AG, Basel

  Author Contacts

Prof. Toshimitsu Niwa, MD
Department of Advanced Medicine for Uremia
Nagoya University Graduate School of Medicine
65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan)
Tel. +81 52 744 1974, Fax +81 52 744 1975, E-Mail tniwa@med.nagoya-u.ac.jp

  Article Information

Received: May 15, 2009
Accepted: July 12, 2009
Published online: August 19, 2009
Number of Print Pages : 6
Number of Figures : 1, Number of Tables : 4, Number of References : 23