Nitric Oxide Metabolites, Leukocyte Activation Markers and Oxidative Status in Dialyzed Subjects

Vol. 27, No. 2, 2009

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Original Paper

Nitric Oxide Metabolites, Leukocyte Activation Markers and Oxidative Status in Dialyzed Subjects
G. Caimi, C. Carollo, M. Montana, R. Iatrino, B. Bondì, R. Lo Presti

Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche, Università di Palermo, Palermo, Italy

Address of Corresponding Author

Blood Purif 2009;27:194-198 (DOI: 10.1159/000193218)

Key Words

Hemodialysis
Oxidative status
Nitric oxide
Elastase
Myeloperoxidase

Abstract

Aims: Our purpose was to evaluate, in a group of 42 end-stage renal disease patients who regularly undergo hemodialysis, some indexes of leukocyte activation, nitric oxide metabolites (NOx) and other parameters that reflect the oxidative stress before and after a standard hemodialysis session. Methods: Elastase and myeloperoxidase (MPO) were determined by means of ELISA. The NO production was evaluated by a micromethod which measures the concentration of NOx. The oxidation of polyunsaturated fatty acids was evaluated in plasma by detection of thiobarbituric acid-reactive substances (TBARS). Total antioxidant status (TAS) was obtained using spectrophotometry. Results: At baseline, we observed an increase of elastase, NOx, TBARS and TAS, without any variation of MPO. After the dialysis session, we found an increase in elastase and MPO, a decrease in NOx and TAS and no variation in TBARS. No modification occurred after subdividing the patients in two subgroups. Conclusions: Our data confirm the involvement of leukocytes and oxidative stress in hemodialysis patients.

Author Contacts

Prof. Gregorio Caimi
Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche
Università di Palermo, Policlinico, Via del Vespro 129
IT-90127 Palermo (Italy)
Fax +39 091 655 4535, E-Mail caimigre@unipa.it

Article Information

Received: July 9, 2008
Accepted: November 4, 2008
Published online: January 20, 2009
Number of Print Pages : 5
Number of Figures : 0, Number of Tables : 2, Number of References : 46

Medline Abstract (ID 19153478)

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