Cytogenetic comparison of saola (Pseudoryx nghetinhensis) and cattle (Bos taurus) using G- and Q-banding and FISHAhrens E.a,b · Graphodatskaya D.a · Nguyen B.X.c · Stranzinger G.a,b
aInstitute of Animal Sciences, Swiss Federal Institute of Technology, Zurich; bVetsuisse Faculty, University of Zurich (Switzerland); cInstitute of Biotechnology, Vietnamese Academy of Sciences and Technology, Hanoi (Vietnam)
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
In order to cytogenetically describe the new bovid species saola (Pseudoryx nghetinhensis), comparative G- and Q-banding of saola and cattle (Bos taurus) chromosomes as well as FISH-mapping of 32 type-I markers (29 Texas markers and three additional markers) on saola chromosomes were performed. Saola was shown to have a diploid number of 2n = 50 chromosomes possessing five biarmed autosomal pairs and an acrocentric X chromosome. Homology of saola and cattle chromosomes was indicated by banding patterns and by marker hybridization suggesting that all five biarmed pairs in saola originate from centric fusions involving ten cattle autosomes. However, small intrachromosomal rearrangements cannot be excluded. In this study the first preliminary homology map of these two species is presented.
© 2005 S. Karger AG, Basel
- Asai M, Graphodatskaya D, Stranzinger G, Joerg H: Assignment of bovine trophinin (TRO) to the q arm of the X chromosome by fluorescence in situ hybridization. Anim Genet 35:142–167 (2004).
- Cribiu EP, Di Berardino D, Di Meo GP, Eggen A, Gallagher DS, Gustavsson I, Hayes H, Iannuzzi L, Popescu CP, Rubes J, Schmutz S, Stranzinger G, Vaiman A, Womack J: International System for Chromosome Nomenclature of Domestic Bovids (ISCNDB 2000). Cytogenet Cell Genet 92:283–299 (2001).
- Di Meo GP, Perucatti A, Gautier M, Hayes H, Incarnato D, Eggen A, Iannuzzi L: Chromosome localization of the 31 type I Texas bovine markers in sheep and goat chromosomes by comparative FISH-mapping and R-banding. Anim Genet 34:294–296 (2003).
- Dung V, Giao P, Chinh N, Tuoc D, Arctander P, MacKinnon J: A new species of living bovid in Vietnam. Nature 363:443–445 (1993).
- Gatesy J, Arctander P: Hidden morphological support for the phylogenetic placement of Pseudoryx nghetinhensis with bovine bovids: A combined analysis of gross anatomical evidence and DNA sequences from five genes. Syst Biol 49:515–538 (2000).
Graphodatskaya D, Perelman P, Serdukova N, Joerg H, Stranzinger G: Assignment of the bovine attractin (ATRN) gene to chromosome 13q21→22 by in situ hybridization. Cytogenet Genome Res 103:204K (2003).
- Hassanin A, Douzery EJ: Evolutionary affinities of the enigmatic saola (Pseudoryx nghetinhensis) in the context of the molecular phylogeny of Bovidae. Proc R Soc Lond B Biol Sci 266:893–900 (1999).
- Hayes H, Di Meo GP, Gautier M, Laurent P, Eggen A, Iannuzzi L: Localization by FISH of the 31 Texas nomenclature type I markers to both Q- and R-banded bovine chromosomes. Cytogenet Cell Genet 90:315–320 (2000).
- Iannuzzi L, Di Meo GP, Hayes H, Perucatti A, Incarnato D, Gautier M, Eggen A: FISH-mapping of 31 type I loci (Texas markers) to river buffalo chromosomes. Chromosome Res 9:339–342 (2001a).
- Iannuzzi L, Di Meo GP, Perucatti A, Schibler L, Incarnato D, Cribiu EP: Comparative FISH-mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes. Cytogenet Cell Genet 94:43–48 (2001b).
- Nguyen TT, Nguyen BX, Stranzinger G: Characterization of G-banded chromosomes of a female saola (Pseudoryx nghetinhensis, 2n = 50) and X chromosome identification by means of fluorescent in situ hybridization (FISH). Cytogenet Genome Res 109:502–506 (2005).
- Schaller G, Rabinowitz A: The saola or spindlehorn bovid Pseudoryx nghetinhensis in Laos. Oryx 29:107–114 (1995).
- Seabright M: A rapid banding technique for human chromosomes. Lancet 2:971–972 (1971).
- Solinas-Toldo S, Lengauer C, Fries R: Comparative genome map of human and cattle. Genomics 27:489–496 (1995).
- Thomas H: Anatomie crânienne et relations phylogénétiques du nouveau bovidé (Pseudoryx nghetinhensis) découvert dans la cordillère annamitique au Vietnam. Mammalia 58:453–481 (1994).
- Toldo SS, Fries R, Steffen P, Neibergs HL, Barendse W, Womack JE, Hetzel DJS, Stranzinger G: Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mamm Genome 4:720–727 (1993).
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.